Biomedical Science and How to Use a Pipette

Biomedical science pipetting
Image: Microscopy open educational resources.
Creative Commons BY SA.

Content Authors:                     Level:

Dr Graham Basten                                 School, College, University, General interest

OER Features: (bundle of 7 resources)

Biomedical science resources: Different Types of Pipette

Biomedical science resources: Pi-Pump Pipette

Biomedical science resources: Micropipettes

Transcript of videos PDF file

Transcript of videos Word file

Multiple choice quiz PDF file

Multiple choice quiz Word file

OER Description:

In the biomedical science laboratory, the pipette is a fundamental piece of equipment that is used routinely for the dispensing of liquids, and pipetting is a skill that takes time to acquire and perfect. There are very few procedures in the laboratory that do not require the addition of a liquid or a solution at some point, from applying stains in histology, to carrying out a spectrophotometer assay.

There are several different types of pipette and each is used in a different situation. You might only require a quick and rough application of a liquid for example to wash a stain off a histology glass slide, so a plastic pipette would suffice. However, if you were performing an assay that was going to give you accurate numbers for the concentration of say glucose in the blood, then a more accurate type of pipette such as a micropipette would be required. Other procedures for example genetic techniques might require the pipetting of tiny amounts would also require very small volume micropipettes to use.

These OERs are a series of 3 videos by Dr Graham Basten explaining the three different type of pipettes and when it is appropriate to use them in the biomedical science laboratory. The other resources include a full transcript of all 3 videos and a short set of multiple choice questions written by Biomedical Science students for release as open educational resources (OERs).

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