Biomedical science laboratory skills resources showing how to use and maintain a light microscope.
Content Authors: Level:
Dr Simon Oldroyd School, College, University, General interest
Dr Vivien Rolfe
OER Features: (bundle of 10 resources)
Biomedical science resources: How to set up a light microscope
PDF and Word & Txt Document Down Loads: Guide to setting up a light microscope.
Biomedical science resources: How to maintain a light microscope.
PDF and Word & Txt Document Down Loads: Guide to maintaining a light microscope.
In the biomedical science laboratory, the light microscope is routinely used to view histology slide samples (that may be obtained from patient biopsies and blood smears) and also to view microbiological samples. This is an important step in the diagnosis of many diseases and specimens will also be analysed to review the progress of various treatments and therapies.
All samples are prepared on a glass slide which is preserved and sealed with a cover slip to enable viewing under the light microscope.
These OERs are a series of 10 resources including videos, accompanying guides and an animation to introduce the light microscope. Also included is a set of quiz questions.
There are several steps that need to be taken to set up the microscope and the first video outlines these to set up the eye pieces and focus the condenser lens and the stage, along with an accompanying PDF and editable Word and Text Document of all the essential steps.
The second video illustrates how a microscope should be cleaned, and for example in our biomedical science student laboratories, all equipment is cleaned and maintained twice a year. There is an accompanying PDF of the audio transcript of this video which again outlines the basic steps. This is also available in editable Word and Text format.
Alongside these resources is an Adobe Flash animation illustrating the parts of the microscope and including a drag and drop quiz to test the user’s understanding.
Understanding the parts, functioning and maintenance of a light microscope is fundamental to any laboratory science student including those studying medical sciences and biomedical science.